RT Journal Article
SR Electronic
T1 Intracellular Optical Doppler Phenotypes of Chemosensitivity in Human Epithelial Ovarian Cancer
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.09.14.296863
DO 10.1101/2020.09.14.296863
A1 Zhe Li
A1 Ran An
A1 Wendy M. Swetzig
A1 Margaux Kanis
A1 Nkechiyere Nwani
A1 John Turek
A1 Daniela Matei
A1 David Nolte
YR 2020
UL http://biorxiv.org/content/early/2020/09/15/2020.09.14.296863.abstract
AB Development of an assay to predict response to chemotherapy has remained an elusive goal in cancer research. We report a phenotypic chemosensitivity assay for epithelial ovarian cancer based on Doppler spectroscopy of infrared light scattered from intracellular motions in living three-dimensional tumor biopsy tissue measured in vitro. The study analyzed biospecimens from 20 human patients with epithelial ovarian cancer. Matched primary and metastatic tumor tissues were collected for 3 patients, and an additional 3 patients provided only metastatic tissues. Doppler fluctuation spectra were obtained using full-field optical coherence tomography through off-axis digital holography. Frequencies in the range from 10 mHz to 10 Hz are sensitive to changes in intracellular dynamics caused by platinum-based chemotherapy. Metastatic tumor tissues were found to display a biodynamic phenotype that was similar to primary tissue from patients who had poor clinical outcomes. The biodynamic phenotypic profile correctly classified 90% [88% to 91% c.i.] of the patients when the metastatic samples were characterized as having a chemoresistant phenotype. This work suggests that Doppler profiling of tissue response to chemotherapy has the potential to predict patient clinical outcomes based on primary, but not metastatic, tumor tissue.Competing Interest StatementDavid Nolte, John Turek and Ran An have a financial interest in Animated Dynamics Inc. which is licensing biodynamic imaging technology from the Office of Technology Commercialization of Purdue University. Zhe Li, Wendy M. Swetzig, Margaux Kanis, Nkechiyere Nwani, and Daniela Matei declare no potential conflict of interest.