RT Journal Article
SR Electronic
T1 An expanded gene catalog of the mouse gut metagenome
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.09.16.299339
DO 10.1101/2020.09.16.299339
A1 Jiahui Zhu
A1 Huahui Ren
A1 Huanzi Zhong
A1 Xiaoping Li
A1 Yuanqiang Zou
A1 Mo Han
A1 Minli Li
A1 Lise Madsen
A1 Karsten Kristiansen
A1 Liang Xiao
YR 2020
UL http://biorxiv.org/content/early/2020/09/16/2020.09.16.299339.abstract
AB High-quality and comprehensive reference gene catalogs are essential for metagenomic research. The rather low diversity of samples used to construct existing catalogs of mouse gut metagenomes limits the size and numbers of identified genes in existing catalogs. We therefore established an expanded gene catalog of genes in the mouse gut metagenomes (EMGC) containing >5.8 million genes by integrating 88 newly sequenced samples, 86 mouse-gut-related bacterial genomes and 3 existing gene catalogs. EMGC increases the number on non-redundant genes by more than one million genes compared to the so far most extensive catalog. More than 50% of the genes in EMGC were taxonomically assigned and 30% were functionally annotated. 902 Metagenomic species (MGS) assigned to 122 taxa are identified based on the EMGC. The EMGC-based analysis of samples from groups of mice originating from different animal providers, housing laboratories and genetic strains substantiated that diet is a major contributor to differences in composition and functional potential of the gut microbiota irrespective of differences in environment and genetic background. We envisage that EMGC will serve as an efficient and resource-saving reference dataset for future metagenomic studies in mice.Competing Interest StatementThe authors have declared no competing interest.