RT Journal Article
SR Electronic
T1 Culturing primary neurons from rat hippocampus and cortex
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 491118
DO 10.1101/491118
A1 Madhusmita Priyadarshini Sahu
A1 Outi Nikkilä
A1 Seija Lågas
A1 Sulo Kolehmainen
A1 Eero Castrén
YR 2019
UL http://biorxiv.org/content/early/2019/01/17/491118.abstract
AB Primary neurons from rodent brain hippocampus and cortex has served as great tools in biomedical research over the years. However, protocols for the preparation of primary neurons vary, which often leads to conflicting results. This report provides a robust and reliable protocol for the production of primary neuronal cultures from the cortex and hippocampus with minimal contribution of non-neuronal cells. The neurons were grown in serum free media and maintained for several weeks without any additional feeder cells. The neuronal cultures maintained according to this protocol differentiate and by three weeks develop extensive axonal and dendritic branching. The cultures produced by this method show excellent reproducibility and can be used for histological, molecular and biochemical methods.