PT  - JOURNAL ARTICLE
AU  - Jo, YoungJu
AU  - Cho, Hyungjoo
AU  - Park, Wei Sun
AU  - Kim, Geon
AU  - Ryu, Donghun
AU  - Kim, Young Seo
AU  - Lee, Moosung
AU  - Joo, Hosung
AU  - Jo, HangHun
AU  - Lee, Sumin
AU  - Min, Hyun-seok
AU  - Heo, Won Do
AU  - Park, YongKeun
TI  - Data-driven multiplexed microtomography of endogenous subcellular dynamics
AID  - 10.1101/2020.09.16.300392
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.09.16.300392
4099  - http://biorxiv.org/content/early/2020/09/18/2020.09.16.300392.short
4100  - http://biorxiv.org/content/early/2020/09/18/2020.09.16.300392.full
AB  - Simultaneous imaging of various facets of intact biological systems across multiple spatiotemporal scales would be an invaluable tool in biomedicine. However, conventional imaging modalities have stark tradeoffs precluding the fulfilment of all functional requirements. Here we propose the refractive index (RI), an intrinsic quantity governing light-matter interaction, as a means for such measurement. We show that major endogenous subcellular structures, which are conventionally accessed via exogenous fluorescence labeling, are encoded in 3D RI tomograms. We decode this information in a data-driven manner, thereby achieving multiplexed microtomography. This approach inherits the advantages of both high-specificity fluorescence imaging and label-free RI imaging. The performance, reliability, and scalability of this technology have been extensively characterized, and its application within single-cell profiling at unprecedented scales has been demonstrated.Competing Interest StatementH.C., M.L., H.-H.J., S.L., H.-S.M., and Y.-K.P. have financial interests in Tomocube Inc., a company that commercializes optical diffraction tomography and quantitative phase imaging instruments and is one of the sponsors of the work.