RT Journal Article
SR Electronic
T1 Data-driven multiplexed microtomography of endogenous subcellular dynamics
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.09.16.300392
DO 10.1101/2020.09.16.300392
A1 Jo, YoungJu
A1 Cho, Hyungjoo
A1 Park, Wei Sun
A1 Kim, Geon
A1 Ryu, Donghun
A1 Kim, Young Seo
A1 Lee, Moosung
A1 Joo, Hosung
A1 Jo, HangHun
A1 Lee, Sumin
A1 Min, Hyun-seok
A1 Heo, Won Do
A1 Park, YongKeun
YR 2020
UL http://biorxiv.org/content/early/2020/09/18/2020.09.16.300392.abstract
AB Simultaneous imaging of various facets of intact biological systems across multiple spatiotemporal scales would be an invaluable tool in biomedicine. However, conventional imaging modalities have stark tradeoffs precluding the fulfilment of all functional requirements. Here we propose the refractive index (RI), an intrinsic quantity governing light-matter interaction, as a means for such measurement. We show that major endogenous subcellular structures, which are conventionally accessed via exogenous fluorescence labeling, are encoded in 3D RI tomograms. We decode this information in a data-driven manner, thereby achieving multiplexed microtomography. This approach inherits the advantages of both high-specificity fluorescence imaging and label-free RI imaging. The performance, reliability, and scalability of this technology have been extensively characterized, and its application within single-cell profiling at unprecedented scales has been demonstrated.Competing Interest StatementH.C., M.L., H.-H.J., S.L., H.-S.M., and Y.-K.P. have financial interests in Tomocube Inc., a company that commercializes optical diffraction tomography and quantitative phase imaging instruments and is one of the sponsors of the work.