RT Journal Article
SR Electronic
T1 A Global Temporal Genetic Program for Neural Circuit Formation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.09.18.304410
DO 10.1101/2020.09.18.304410
A1 Jain, Saumya
A1 Lin, Ying
A1 Kurmangaliyev, Yerbol
A1 Mirshahidi, Parmis
A1 Parrington, Brianna
A1 Zipursky, S. Lawrence
YR 2020
UL http://biorxiv.org/content/early/2020/09/19/2020.09.18.304410.abstract
AB Wiring a complex brain relies on cell-type and temporal-specific expression of genes encoding cell recognition molecules regulating circuit formation1–3. Though genetic programs driving cell-type specificity have been described4–6, how precise temporal control is achieved remains unknown. Here, we describe a global program for the temporal regulation of cell-type-specific wiring genes in the Drosophila visual system. We show that the Ecdysone Receptor induces a synchronous cascade of transcription factors in neurons throughout the visual system in a highly stereotyped fashion. Single-cell RNA-seq analysis of neurons lacking transcription factors in the cascade revealed that targets are cell-type dependent and these are enriched for wiring genes. Knock-down of different transcription factors in this cascade led to defects in sequential steps in wiring. Taken together, this work identifies a synchronous, global program for temporal control of different sets of wiring genes in different neurons. We speculate that this global program coordinates development across cell types to choreograph specific steps in circuit assembly.Competing Interest StatementThe authors have declared no competing interest.