@article {Kim522383, author = {Keun-Tae Kim and Ju-Chan Park and Haeseung Lee and Hyeon-Ki Jang and Yan Jin and Wankyu Kim and Jeongmi Lee and Hyongbum Henry Kim and Sang-Su Bae and Hyuk-Jin Cha}, title = {Scarless Enriched selection of Genome edited Human Pluripotent Stem Cells Using Induced Drug Resistance}, elocation-id = {522383}, year = {2019}, doi = {10.1101/522383}, publisher = {Cold Spring Harbor Laboratory}, abstract = {An efficient gene editing technique for use in human pluripotent stem cells (hPSCs) would have great potential value in regenerative medicine, as well as in drug discovery based on isogenic human disease models. However, the extremely low efficiency of gene editing in hPSCs is a major technical hurdle that remains to be resolved. Previously, we demonstrated that YM155, a survivin inhibitor developed as an anti-cancer drug, induces highly selective cell death in undifferentiated hPSCs. In this study, we demonstrated that the high cytotoxicity of YM155 in hPSCs, which is mediated by selective cellular uptake of the drug, is due to high expression of SLC35F2 in these cells. Consistent with this, knockout of SLC35F2 with CRISPR-Cas9 or depletion with siRNAs made hPSCs highly resistant to YM155. Simultaneous gene editing of a gene of interest and transient knockdown of SLC35F2 following YM155 treatment enabled genome-edited hPSCs to survive because YM155 resistance was temporarily induced, thereby achieving enriched selection of genome-edited clonal populations. This precise and efficient genome editing approach took as little as 3 weeks without cell sorting or introduction of additional genes.}, URL = {https://www.biorxiv.org/content/early/2019/01/18/522383}, eprint = {https://www.biorxiv.org/content/early/2019/01/18/522383.full.pdf}, journal = {bioRxiv} }