PT  - JOURNAL ARTICLE
AU  - Heesun Kim
AU  - Yue-He Ding
AU  - Shan Lu
AU  - Mei-Qing Zuo
AU  - Darryl Conte, Jr.
AU  - Meng-Qiu Dong
AU  - Craig C. Mello
TI  - PIE-1 promotes SUMOylation and activation of HDAC1 during the <em>C. elegans</em> oogenesis
AID  - 10.1101/2020.08.17.253955
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.08.17.253955
4099  - http://biorxiv.org/content/early/2020/09/19/2020.08.17.253955.short
4100  - http://biorxiv.org/content/early/2020/09/19/2020.08.17.253955.full
AB  - Germlines shape and balance heredity, integrating and regulating information from both parental and foreign sources. Insights into how the germline handles information have come from the identification of factors that specify or maintain the germline fate. In early C. elegans embryos, the CCCH zinc-finger protein PIE-1 localizes to the germline where it prevents somatic differentiation programs. Here we show that PIE-1 also functions in the meiotic ovary where it becomes SUMOylated and engages the SUMO-conjugating machinery. Using whole-proteome mass spectrometry to detect SUMO-conjugated proteins, we identify HDAC SUMOylation as a target of PIE-1. Our findings suggest that SUMOylation activates HDAC, lowering histone acetylation and enhancing Argonaute-mediated surveillance in the germline.Competing Interest StatementThe authors have declared no competing interest.