RT Journal Article
SR Electronic
T1 HDAC1 SUMOylation promotes Argonaute directed transcriptional silencing in <em>C. elegans</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.08.17.254466
DO 10.1101/2020.08.17.254466
A1 Heesun Kim
A1 Yue-He Ding
A1 Gangming Zhang
A1 Yong-Hong Yan
A1 Darryl Conte, Jr.
A1 Meng-Qiu Dong
A1 Craig C. Mello
YR 2020
UL http://biorxiv.org/content/early/2020/09/19/2020.08.17.254466.abstract
AB Eukaryotic cells use guided search to coordinately control dispersed genetic elements. The transitive effectors of these mechanisms, Argonaute proteins and their small-RNA co-factors, engage nascent RNAs and chromatin-associated proteins to direct transcriptional silencing. The small ubiquitin-like modifier (SUMO) has been shown to promote the induction and maintenance of silent chromatin (called heterochromatin) in yeast, plants, and animals. Here we show that Argonaute-directed transcriptional silencing in C. elegans requires SUMOylation of the type 1 histone deacetylase HDA-1. SUMOylation of HDA-1 promotes interactions with components of the nucleosome remodeling and deacetylase (NuRD) complex and with the nuclear Argonaute HRDE-1/WAGO-9. Our findings suggest how HDAC1 SUMOylation promotes the association of HDAC and other chromatin remodeling factors with a nuclear Argonaute in order to initiate de novo heterochromatin silencing.Competing Interest StatementThe authors have declared no competing interest.