TY - JOUR T1 - <em>In vivo</em> structure of the <em>Legionella</em> type II secretion system by electron cryotomography JF - bioRxiv DO - 10.1101/525063 SP - 525063 AU - Debnath Ghosal AU - Ki Woo Kim AU - Huaixin Zheng AU - Mohammed Kaplan AU - Joseph P. Vogel AU - Nicholas P. Cianciotto AU - Grant J. Jensen Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/01/19/525063.abstract N2 - The type II secretion system (T2SS) is a multi-protein envelope-spanning assembly that translocates a wide range of virulence factors, enzymes and effectors through the outer membrane (OM) of many Gram-negative bacteria. Here, using electron cryotomography and subtomogram averaging methods, we present the first in situ structure of an intact T2SS, imaged within the human pathogen Legionella pneumophila. Although the T2SS has only limited sequence and component homology with the evolutionarily-related Type IV pilus (T4P) system, we show that their overall architectures are remarkably similar. Despite similarities, there are also differences, including for instance that the T2SS-ATPase complex is usually present but disengaged from the inner membrane, the T2SS has a much longer periplasmic vestibule, and it has a short-lived flexible pseudopilus. Placing atomic models of the components into our ECT map produced a complete architectural model of the intact T2SS that provides new insights into the structure and function of its components, its position within the cell envelope, and the interactions between its different subcomplexes. Overall, these structural results strongly support the piston model for substrate extrusion. ER -