RT Journal Article
SR Electronic
T1 Fibrinogen anchors for micropatterning of active proteins and subcellular receptor relocalisation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.09.04.256875
DO 10.1101/2020.09.04.256875
A1 Joseph L. Watson
A1 Samya Aich
A1 Benjamí Oller Salvia
A1 Andrew A. Drabek
A1 Stephen C. Blacklow
A1 Jason Chin
A1 Emmanuel Derivery
YR 2020
UL http://biorxiv.org/content/early/2020/09/21/2020.09.04.256875.abstract
AB Protein micropatterning allows proteins to be precisely deposited onto a substrate of choice, and is now routinely used in cell biology and in vitro reconstitution. However, a drawback of current technology is that micropatterning efficiency can be variable between proteins, and that proteins may lose activity on the micropatterns. Here, we describe a general method to enable micropatterning of virtually any protein at high specificity and homogeneity while maintaining its activity. Our method is based on an anchor that micropatterns well, Fibrinogen, which we functionalized to bind to common purification tags. This enhances micropatterning on various substrates, facilitates multiplexed micropatterning, and dramatically improves the on-pattern activity of fragile proteins like molecular motors. Furthermore, it enhances the micropatterning of hard to micropattern cells. Last, this method enables subcellular micropatterning, whereby complex micropatterns simultaneously control cell shape and the distribution of transmembrane receptors within that cell. Altogether, these results open new avenues for cell biology.Competing Interest StatementThe authors have declared no competing interest.