PT  - JOURNAL ARTICLE
AU  - Huanle Liu
AU  - Oguzhan Begik
AU  - Morghan C Lucas
AU  - Christopher E. Mason
AU  - Schraga Schwartz
AU  - John S. Mattick
AU  - Martin A. Smith
AU  - Eva Maria Novoa
TI  - Accurate detection of m6A RNA modifications in native RNA sequences
AID  - 10.1101/525741
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 525741
4099  - http://biorxiv.org/content/early/2019/01/21/525741.short
4100  - http://biorxiv.org/content/early/2019/01/21/525741.full
AB  - The field of epitranscriptomics has undergone an enormous expansion in the last few years; however, a major limitation is the lack of generic methods to map RNA modifications transcriptome-wide. Here we show that using Oxford Nanopore Technologies, N6-methyladenosine (m6A) RNA modifications can be detected with high accuracy, in the form of systematic errors and decreased base-calling qualities. Our results open new avenues to investigate the universe of RNA modifications with single nucleotide resolution, in individual RNA molecules.(m6A)N6-methlyadenosine(m1A)N1-methyladenosine(5hmc)5-hydroxymethylcytosine(ONT)Oxford Nanopore Technologies(SVM)Support Vector Machine(IVT)In vitro transcription