RT Journal Article
SR Electronic
T1 Accurate detection of m6A RNA modifications in native RNA sequences
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 525741
DO 10.1101/525741
A1 Huanle Liu
A1 Oguzhan Begik
A1 Morghan C Lucas
A1 Christopher E. Mason
A1 Schraga Schwartz
A1 John S. Mattick
A1 Martin A. Smith
A1 Eva Maria Novoa
YR 2019
UL http://biorxiv.org/content/early/2019/01/21/525741.abstract
AB The field of epitranscriptomics has undergone an enormous expansion in the last few years; however, a major limitation is the lack of generic methods to map RNA modifications transcriptome-wide. Here we show that using Oxford Nanopore Technologies, N6-methyladenosine (m6A) RNA modifications can be detected with high accuracy, in the form of systematic errors and decreased base-calling qualities. Our results open new avenues to investigate the universe of RNA modifications with single nucleotide resolution, in individual RNA molecules.(m6A)N6-methlyadenosine(m1A)N1-methyladenosine(5hmc)5-hydroxymethylcytosine(ONT)Oxford Nanopore Technologies(SVM)Support Vector Machine(IVT)In vitro transcription