RT Journal Article
SR Electronic
T1 Chicdiff: a computational pipeline for detecting differential chromosomal interactions in Capture Hi-C data
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 526269
DO 10.1101/526269
A1 Jonathan Cairns
A1 William R. Orchard
A1 Valeriya Malysheva
A1 Mikhail Spivakov
YR 2019
UL http://biorxiv.org/content/early/2019/01/22/526269.abstract
AB Capture Hi-C is a powerful approach for detecting chromosomal interactions involving, at least on one end, DNA regions of interest, such as gene promoters. We present Chicdiff, an R package for robust detection of differential interactions in Capture Hi-C data. Chicdiff enhances a state-of-the-art differential testing approach for count data with bespoke normalisation and multiple testing procedures that account for specific statistical properties of Capture Hi-C. We validate Chicdiff on published Promoter Capture Hi-C data in human monocytes and CD4+ T cells, identifying multitudes of cell type-specific interactions, and confirming the overall positive association between promoter interactions and gene expression.