RT Journal Article
SR Electronic
T1 A chromosome-level genome of black rockfish, Sebastes schlegelii, provides insights into the evolution of live birth
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 527036
DO 10.1101/527036
A1 Yan He
A1 Yue Chang
A1 Lisui Bao
A1 Mengjun Yu
A1 Rui Li
A1 Jingjing Niu
A1 Guangyi Fan
A1 Weihao Song
A1 Inge Seim
A1 Yating Qin
A1 Xuemei Li
A1 Jinxiang Liu
A1 Xiangfu Kong
A1 Meiting Peng
A1 Minmin Sun
A1 Mengya Wang
A1 Jiangbo Qu
A1 Xuangang Wang
A1 Xiaobing Liu
A1 Xiaolong Wu
A1 Xi Zhao
A1 Xuliang Wang
A1 Yaolei Zhao
A1 Jiao Guo
A1 Yang Liu
A1 Kaiqiang Liu
A1 Yilin Wang
A1 He Zhang
A1 Longqi Liu
A1 Mingyue Wang
A1 Haiyang Yu
A1 Xubo Wang
A1 Jie Cheng
A1 Zhigang Wang
A1 Xun Xu
A1 Jian Wang
A1 Huanming Yang
A1 Simon Ming-Yuen Lee
A1 Xin Liu
A1 Quanqi Zhang
A1 Jie Qi
YR 2019
UL http://biorxiv.org/content/early/2019/01/22/527036.abstract
AB Black rockfish (Sebastes schlegelii) is a teleost species where eggs are fertilized internally and retained in the maternal reproductive system, where they undergo development until live birth (termed viviparity). In the present study, we report a chromosome-level black rockfish genome assembly. High-throughput transcriptome analysis (RNA-seq and ATAC-seq), coupled with in situ hybridization (ISH) and immunofluorescence, identify several candidate genes for maternal preparation, sperm storage and release, and hatching. We propose that zona pellucida (ZP) genes retain sperm at the oocyte envelope, while genes in two distinct astacin metalloproteinase subfamilies serve to release sperm from the ZP and free the embryo from chorion at pre-hatching stage. Finally, we present a model of black rockfish reproduction, and propose that the rockfish ovarian wall has a similar function to uterus of mammals. Taken together, these genomic data reveal unprecedented insights into the evolution of an unusual teleost life history strategy, and provide a sound foundation for studying viviparity in non-mammalian vertebrates and an invaluable resource for rockfish ecology and evolution research.