PT  - JOURNAL ARTICLE
AU  - Kurataka Otsuka
AU  - Akihito Mamiya
AU  - Mineko Konishi
AU  - Mamoru Nozaki
AU  - Atsuko Kinoshita
AU  - Hiroaki Tamaki
AU  - Masaki Arita
AU  - Masato Saito
AU  - Kayoko Yamamoto
AU  - Takushi Hachiya
AU  - Ko Noguchi
AU  - Takashi Ueda
AU  - Yusuke Yagi
AU  - Takehito Kobayashi
AU  - Takahiro Nakamura
AU  - Yasushi Sato
AU  - Takashi Hirayama
AU  - Munetaka Sugiyama
TI  - Temperature-dependent fasciation mutants connect mitochondrial RNA processing to control of lateral root morphogenesis
AID  - 10.1101/2020.06.09.141382
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.06.09.141382
4099  - http://biorxiv.org/content/early/2020/09/23/2020.06.09.141382.short
4100  - http://biorxiv.org/content/early/2020/09/23/2020.06.09.141382.full
AB  - Although mechanisms that activate organogenesis in plants are well established, much less is known about the subsequent fine-tuning of cell proliferation, which is crucial for creating properly structured and sized organs. Here we show, through analysis of temperature-dependent fasciation (TDF) mutants of Arabidopsis, root redifferentiation defective 1 (rrd1), rrd2, and root initiation defective 4 (rid4), that mitochondrial RNA processing is required for limiting cell division during early lateral root (LR) organogenesis. These mutants formed abnormally broadened (i.e., fasciated) LRs under high-temperature conditions due to excessive cell division. All TDF proteins localized to mitochondria, where they were found to participate in RNA processing: RRD1 in mRNA deadenylation, and RRD2 and RID4 in mRNA editing. Further analysis suggested that LR fasciation in the TDF mutants is triggered by reactive oxygen species generation caused by defective mitochondrial respiration. Our findings provide novel clues for the physiological significance of mitochondrial activities in plant organogenesis.Competing Interest StatementThe authors have declared no competing interest.