RT Journal Article
SR Electronic
T1 YAP/TAZ as a Novel Regulator of cell volume
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 528133
DO 10.1101/528133
A1 Nicolas A. Perez-Gonzalez
A1 Nash D. Rochman
A1 Kai Yao
A1 Jiaxiang Tao
A1 Minh-Tam Tran Le
A1 Shannon Flanary
A1 Lucia Sablich
A1 Ben Toler
A1 Eliana Crentsil
A1 Felipe Takaesu
A1 Bram Lambrus
A1 Jessie Huang
A1 Vivian Fu
A1 Andrew J. Holland
A1 Steven An
A1 Denis Wirtz
A1 Kun-Liang Guan
A1 Sean X. Sun
YR 2019
UL http://biorxiv.org/content/early/2019/01/23/528133.abstract
AB How mammalian cells regulate their physical size is currently poorly understood, in part due to the difficulty of accurately quantifying cell volume in a high throughput manner. Here, using the fluorescence exclusion method, we demonstrate that the mechanosensitive transcriptional regulators YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) are novel regulators of single cell volume. We report that the role of YAP/TAZ in cell volume regulation must go beyond its influence on total cell cycle duration or the cell shape to explain the observed changes in volume. Moreover, for our experimental conditions, volume regulation by YAP/TAZ is independent of mTOR. Instead, we find YAP/TAZ directly impacts the cell division volume. Based on the idea that YAP/TAZ is a mechanosensor, we find that inhibiting the assembly of myosin and cell tension slows cell cycle progression from G1 to S. These results suggest that YAP/TAZ and the Hippo pathway may be modulating cell volume in combination with cytoskeletal tension during cell cycle progression.