PT  - JOURNAL ARTICLE
AU  - Anabel-Lise Le Roux
AU  - Caterina Tozzi
AU  - Nikhil Walani
AU  - Xarxa Quiroga
AU  - Dobryna Zalvidea
AU  - Xavier Trepat
AU  - Margarita Staykova
AU  - Marino Arroyo
AU  - Pere Roca-Cusachs
TI  - Dynamic Mechanochemical feedback between curved membranes and BAR protein self-organization
AID  - 10.1101/2020.09.23.310169
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.09.23.310169
4099  - http://biorxiv.org/content/early/2020/09/25/2020.09.23.310169.short
4100  - http://biorxiv.org/content/early/2020/09/25/2020.09.23.310169.full
AB  - In many physiological situations, BAR proteins interact with, and reshape, pre-existing curved membranes, contributing to essential cellular processes. However, the non-equilibrium and timedependent process of reshaping, and its dependence on initial membrane shape, remains largely unknown. Here we explain, both experimentally and through modelling, how a BAR protein dynamically interacts with mechanically bent lipid membranes. We capture protein binding to curved membranes, and characterize a variety of dynamical reshaping events depending on membrane shape and protein arrangement. The events can be generally understood by an isotropic-to-nematic phase transition, in which low curvature templates with isotropic protein orientation progress towards highly curved lipid tubes with nematic protein arrangement. Our findings also apply in cells, where mechanical stretch triggers BAR-protein-membrane interactions that enable potential mechanotransduction mechanisms. Our results characterize and broaden the reshaping processes of BAR proteins on mechanically constrained membranes, demonstrating the interplay between membrane mechanical stimuli and BAR protein response.Competing Interest StatementThe authors have declared no competing interest.