RT Journal Article
SR Electronic
T1 Members of the Arabidopsis auxin receptor gene family are essential early in embryogenesis and have broadly overlapping functions
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 529248
DO 10.1101/529248
A1 Michael J. Prigge
A1 Nikita Kadakia
A1 Kathleen Greenham
A1 Mark Estelle
YR 2019
UL http://biorxiv.org/content/early/2019/01/23/529248.abstract
AB The TIR1/AFB proteins are auxin co-receptors that mediate diverse responses to the plant hormone auxin. The genome of Arabidopsis encodes six TIR1/AFB proteins representing three of the four clades that were established prior to angiosperm radiation. To determine the role of these proteins in plant growth and development we have performed an extensive genetic analysis involving the generation and characterization of all combinations of multiply mutant lines. We find that loss of all six proteins results in defects in embryogenesis as early as the first division of the apical cell. Mutant embryos progress, but exhibit frequent errors in cell division and proliferation of the suspensor. Despite this dramatic phenotype, a single wild-type allele of TIR1 or AFB2 is sufficient to support growth of the plant throughout vegetative development. Further, the TIR1/AFB pathway does not appear to be essential for development of the male or female gametophyte. Our analysis has revealed extensive functional overlap between even the most distantly related TIR1/AFB genes as well as examples of specialization.