RT Journal Article
SR Electronic
T1 Parallel control of mechanosensory hair cell orientation by the PCP and Wnt pathways
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 527937
DO 10.1101/527937
A1 Joaquin Navajas Acedo
A1 Matthew G. Voas
A1 Richard Alexander
A1 Thomas Woolley
A1 Jay R. Unruh
A1 Hua Li
A1 Cecilia Moens
A1 Tatjana Piotrowski
YR 2019
UL http://biorxiv.org/content/early/2019/01/23/527937.abstract
AB Cell polarity plays a crucial role during development of vertebrates and invertebrates. Planar Cell Polarity (PCP) is defined as the coordinated polarity of cells within a tissue axis and is essential for processes such as gastrulation, neural tube closure or hearing. Wnt ligands can be instructive or permissive during PCP-dependent processes, and Wnt pathway mutants are often classified as PCP mutants due to the complexity and the similarities between their phenotypes. Our studies of the zebrafish sensory lateral line reveal that disruptions of the PCP and Wnt pathways have differential effects on hair cell orientations. While mutations in PCP genes cause random orientations of hair cells, mutations in Wnt pathway members induce hair cells to adopt a concentric pattern. We show that PCP signaling is normal in hair cells of Wnt pathway mutants and that the concentric hair cell phenotype is due to altered organization of the surrounding support cells. Thus, the PCP and Wnt pathways work in parallel, as separate pathways to establish proper hair cell orientation. Our data suggest that coordinated support cell organization is established during the formation of lateral line primordia, much earlier than the appearance of hair cells. Together, these finding reveal that hair cell orientation defects are not solely explained by defects in PCP signaling and that some hair cell phenotypes warrant reevaluation.