PT  - JOURNAL ARTICLE
AU  - Junaid Akhtar
AU  - Piyush More
AU  - Steffen Albrecht
AU  - Federico Marini
AU  - Waldemar Kaiser
AU  - Apurva Kulkarni
AU  - Leszek Wojnowski
AU  - Jean-Fred Fontaine
AU  - Miguel A. Andrade-Navarro
AU  - Marion Silies
AU  - Christian Berger
TI  - TAF-ChIP: An ultra-low input approach for genome wide chromatin immunoprecipitation assay
AID  - 10.1101/299727
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 299727
4099  - http://biorxiv.org/content/early/2019/01/24/299727.short
4100  - http://biorxiv.org/content/early/2019/01/24/299727.full
AB  - Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is powerful technique to study transcriptional regulation. However, the requirement of millions of cells to generate results with high signal-to-noise ratio precludes it in the study of small cell populations. Here, we present a Tagmentation-Assisted Fragmentation ChIP (TAF-ChIP) and sequencing method to generate high-quality results from low cell numbers. The data obtained from the TAF-ChIP approach is amenable to standard tools for ChIP-Seq analysis, owing to its high signal-to-noise ratio. The epigenetic profiles from TAF-ChIP approach showed high agreement with conventional ChIP-Seq datasets, thereby underlining the utility of this approach.