RT Journal Article
SR Electronic
T1 TAF-ChIP: An ultra-low input approach for genome wide chromatin immunoprecipitation assay
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 299727
DO 10.1101/299727
A1 Junaid Akhtar
A1 Piyush More
A1 Steffen Albrecht
A1 Federico Marini
A1 Waldemar Kaiser
A1 Apurva Kulkarni
A1 Leszek Wojnowski
A1 Jean-Fred Fontaine
A1 Miguel A. Andrade-Navarro
A1 Marion Silies
A1 Christian Berger
YR 2019
UL http://biorxiv.org/content/early/2019/01/24/299727.abstract
AB Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is powerful technique to study transcriptional regulation. However, the requirement of millions of cells to generate results with high signal-to-noise ratio precludes it in the study of small cell populations. Here, we present a Tagmentation-Assisted Fragmentation ChIP (TAF-ChIP) and sequencing method to generate high-quality results from low cell numbers. The data obtained from the TAF-ChIP approach is amenable to standard tools for ChIP-Seq analysis, owing to its high signal-to-noise ratio. The epigenetic profiles from TAF-ChIP approach showed high agreement with conventional ChIP-Seq datasets, thereby underlining the utility of this approach.