PT  - JOURNAL ARTICLE
AU  - Fang Xu
AU  - Yan Shen
AU  - Lufeng Ding
AU  - Chao-Yu Yang
AU  - Heng Tan
AU  - Hao Wang
AU  - Qingyuan Zhu
AU  - Rui Xu
AU  - Fengyi Wu
AU  - Cheng Xu
AU  - Qianwei Li
AU  - Peng Su
AU  - Li I. Zhang
AU  - Hongwei Dong
AU  - Robert Desimone
AU  - Fuqiang Xu
AU  - Xintian Hu
AU  - Pak-Ming Lau
AU  - Guo-Qiang Bi
TI  - High-throughput whole-brain mapping of rhesus monkey at micron resolution
AID  - 10.1101/2020.09.25.313395
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.09.25.313395
4099  - http://biorxiv.org/content/early/2020/09/26/2020.09.25.313395.short
4100  - http://biorxiv.org/content/early/2020/09/26/2020.09.25.313395.full
AB  - Whole-brain mesoscale mapping of primates has been hindered by large brain size and the relatively low throughput of available microscopy methods. Here, we present an integrative approach that combines primate-optimized tissue sectioning and clearing with ultrahigh-speed, large-scale, volumetric fluorescence microscopy, capable of completing whole-brain imaging of a rhesus monkey at 1 µm × 1 µm × 2.5 µm voxel resolution within 100 hours. A progressive strategy is developed for high-efficiency, long-range tracing of individual axonal fibers through the dataset of hundreds of terabytes, establishing a “Serial sectioning and clearing, 3-dimensional Microscopy, with semi-Automated Reconstruction and Tracing” (SMART) pipeline. This system supports effective connectome-scale mapping of large primates that reveals distinct features of thalamocortical projections of the rhesus monkey brain at the level of individual axonal fibers.Competing Interest StatementThe authors have declared no competing interest.