RT Journal Article
SR Electronic
T1 RNA interactions with CTCF are essential for its proper function
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 530014
DO 10.1101/530014
A1 Ricardo Saldaña-Meyer
A1 Javier Rodriguez-Hernaez
A1 Mayilaadumveettil Nishana
A1 Karina Jácome-López
A1 Elphege P. Nora
A1 Benoit G. Bruneau
A1 Mayra Furlan-Magaril
A1 Jane Skok
A1 Danny Reinberg
YR 2019
UL http://biorxiv.org/content/early/2019/01/24/530014.abstract
AB The function of the CCCTC-binding factor (CTCF) in the organization of the genome has become an important area of investigation, but the mechanisms of how CTCF dynamically contributes to genome organization is not clear. We previously discovered that CTCF binds to large numbers of endogenous RNAs; promoting its oligomerization. Here we found that inhibition of transcription or interfering with CTCF ability to bind RNA through mutations of two of its 11 zinc fingers that are not involved with CTCF binding to its cognate site in vitro, zinc finger-1 (ZF1) or −10 (ZF10), disrupt CTCF association to chromatin. These mutations alter gene expression profiles as CTCF mutants lose their ability to promote local insulation. Our results highlight the importance of RNA as a structural component of the genome, in part by affecting the association of CTCF with chromatin and likely its interaction with other factors.Transcriptional inhibition disrupts CTCF binding to chromatinRNA-binding regions (RBR) in CTCF are found within ZF1 and ZF10Local insulation is markedly decreased in ZF1∆ and ZF10∆ mutant rescuesGene expression and chromatin organization are disrupted by RBR mutants