PT  - JOURNAL ARTICLE
AU  - Wei Dai
AU  - Xiaoran Guo
AU  - Yuansheng Cao
AU  - James A. Mondo
AU  - Joseph P. Campanale
AU  - Brandon J. Montell
AU  - Haley Burrous
AU  - Sebastian Streichan
AU  - Nir Gov
AU  - Wouter Jan Rappel
AU  - Denise J. Montell
TI  - Tissue topography steers migrating Drosophila border cells
AID  - 10.1101/2020.09.27.316117
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.09.27.316117
4099  - http://biorxiv.org/content/early/2020/09/28/2020.09.27.316117.short
4100  - http://biorxiv.org/content/early/2020/09/28/2020.09.27.316117.full
AB  - Moving cells can sense and respond to physical features of the microenvironment, however in vivo the significance of tissue topography is mostly unknown. Here we use the Drosophila border cells, an established model for in vivo cell migration, to study how chemical and physical information influence migration path selection. Live imaging, genetics, modeling, and simulations show that, although chemical cues were thought to be sufficient, microtopography is also important. Chemoattractants promote predominantly posterior movement, whereas tissue architecture presents orthogonal information, a path of least resistance concentrated near the center of the egg chamber. E-cadherin supplies a permissive haptotactic cue. Our results provide insight into how cells integrate and prioritize topographical, adhesive, and chemoattractant cues to choose one path amongst many.Competing Interest StatementThe authors have declared no competing interest.