RT Journal Article SR Electronic T1 Visualization and analysis of high-throughput in vitro dose–response datasets with Thunor JF bioRxiv FD Cold Spring Harbor Laboratory SP 530329 DO 10.1101/530329 A1 Alexander L. R. Lubbock A1 Leonard A. Harris A1 Vito Quaranta A1 Darren R. Tyson A1 Carlos F. Lopez YR 2019 UL http://biorxiv.org/content/early/2019/01/25/530329.abstract AB Quantifying the effects of drugs and other environmental factors on cell proliferation in vitro continues to be one of the most prevalent assays in biomedical research. Assessment of the dose-dependent nature of drug effects is typically performed with a variety of commercial software applications or using freely available, but more technically demanding, statistical programming environments such as Python or R. However, with the advent of large, publicly-available drug response databases and continued advancements in high-throughput experimentation, there is a growing need for user-friendly software platforms that can efficiently and reliably facilitate analysis within and across large datasets. Here we introduce Thunor, an open-source software platform for the management, analysis, and visualization of large-scale dose-dependent cell proliferation datasets. Thunor provides a simple, user-friendly interface to upload cell count data and a graphical plate map tool to annotate plate wells with cell lines and drugs. Best-fit dose–response curves are generated based on either cell viability or proliferation rate drug effect metrics. Derived dose–response parameters, such as IC50, Emax, and activity area, are automatically calculated by the software back-end. An arrayed plot interface supports multiple plot types, including time course, dose–response curve, box/bar/scatter plots of derived parameters, and quality control analyses, among others. We demonstrate the features of Thunor on large-scale, publicly-available viability data and an in-house, high-throughput proliferation rate dataset. Software, documentation, and an online demo are all available at thunor.net.