RT Journal Article
SR Electronic
T1 Identification of TMEM106B as proviral host factor for SARS-CoV-2
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.09.28.316281
DO 10.1101/2020.09.28.316281
A1 Jim Baggen
A1 Leentje Persoons
A1 Sander Jansen
A1 Els Vanstreels
A1 Maarten Jacquemyn
A1 Dirk Jochmans
A1 Johan Neyts
A1 Kai Dallmeier
A1 Piet Maes
A1 Dirk Daelemans
YR 2020
UL http://biorxiv.org/content/early/2020/09/28/2020.09.28.316281.abstract
AB The ongoing COVID-19 pandemic is responsible for worldwide economic damage and nearly one million deaths. Potent drugs for the treatment of severe SARS-CoV-2 infections are not yet available. To identify host factors that support coronavirus infection, we performed genome-wide functional genetic screens with SARS-CoV-2 and the common cold virus HCoV-229E in non-transgenic human cells. These screens identified PI3K type 3 as a potential drug target against multiple coronaviruses. We discovered that the lysosomal protein TMEM106B is an important host factor for SARS-CoV-2 infection. Furthermore, we show that TMEM106B is required for replication in multiple human cell lines derived from liver and lung and is expressed in relevant cell types in the human airways. Our results identify new coronavirus host factors that may potentially serve as drug targets against SARS-CoV-2 or to quickly combat future zoonotic coronavirus outbreaks.Competing Interest StatementThe authors have declared no competing interest.