PT  - JOURNAL ARTICLE
AU  - Shuqin Zhou
AU  - Siyu Feng
AU  - David Brown
AU  - Bo Huang
TI  - Improved yellow-green split fluorescent proteins for protein labeling and signal amplification
AID  - 10.1101/2020.09.27.315697
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.09.27.315697
4099  - http://biorxiv.org/content/early/2020/09/28/2020.09.27.315697.short
4100  - http://biorxiv.org/content/early/2020/09/28/2020.09.27.315697.full
AB  - The flexibility and versatility of self-complementing split fluorescent proteins (FPs) have enabled a wide range of applications. In particular, the FP1-10/11 split system contains a small fragment that facilitates efficient generation of endogenous-tagged cell lines and animals as well as signal amplification using tandem FP11 tags. To improve the FP1-10/11 toolbox we previously developed, here we used a combination of directed evolution and rational design approaches, resulting in two mNeonGreen (mNG)-based split FPs (mNG3A1-10/11 and mNG3K1-10/11) and one mClover-based split FP (CloGFP1-10/11). mNG3A1-10/11 and mNG3K1-10/11 not only enhanced the complementation efficiency at low expression levels, but also allowed us to demonstrate signal amplification using tandem mNG211 fragments in mammalian cells.Competing Interest StatementThe authors have declared no competing interest.