RT Journal Article
SR Electronic
T1 Rice bran supplementation modulates growth, microbiome and metabolome in weaning infants: a clinical trial in Nicaragua and Mali
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 530089
DO 10.1101/530089
A1 Luis E. Zambrana
A1 Starin McKeen
A1 Hend Ibrahim
A1 Iman Zarei
A1 Erica C. Borresen
A1 Lassina Doumbia
A1 Abdoulaye Bore
A1 Alima Cissoko
A1 Seydou Douyon
A1 Karim Kone
A1 Johann Perez
A1 Claudia Perez
A1 Ann Hess
A1 Zaid Abdo
A1 Lansana Sangare
A1 Ababacar Maiga
A1 Sylvia Becker-Dreps
A1 Lijuan Yuan
A1 Ousmane Koita
A1 Samuel Vilchez
A1 Elizabeth P. Ryan
YR 2019
UL http://biorxiv.org/content/early/2019/01/26/530089.abstract
AB Rice bran supplementation provides nutrients, prebiotics and phytochemicals that enhance gut immunity, reduce enteric pathogens in mice and diarrhea in neonatal pigs, and warranted attention for improvement of environmental enteric dysfunction (EED) in children at risk. EED is a condition that drives childhood stunting via intestinal dysbiosis and impaired nutrient metabolism. This study investigated effects of rice bran supplementation on growth, EED biomarkers, gut microbiome and metabolome in weaning infants from 6 to 12 months old in Nicaragua and Mali. Healthy infants were randomized to a control group or rice bran group that received daily supplementation at increasing doses each month. Stool microbiomes were characterized using 16S rDNA amplicon sequencing. Stool metabolomes were analyzed using ultra-high-performance liquid-chromatography tandem mass-spectrometry. Statistical comparisons were completed at 6, 8, and 12 months of age. Daily consumption of rice bran was safe and feasible for infant growth, decreasing alpha-1 antitrypsin levels, and modulating gut microbiome and metabolome when compared to control. Rice bran merits investigation as a practical intervention strategy that could decrease EED prevalence and risk for children from low- and middle-income countries where rice is grown as a staple food, and bran is used as animal feed or wasted.One Sentence Summary Dietary rice bran supplementation during infant weaning from 6-12 months of age improved growth outcomes, modulated environmental enteric dysfunction biomarkers, and supported metabolism by the gut microbiome.The authors declare no competing financial or non financial interests to disclose as defined by Nature Research. There are also no other interests that might be perceived to influence the results and/or discussion reported in this paper.Correspondence and requests for materials should be addressed to Dr. Elizabeth Ryan (e.p.ryan{at}colostate.edu).