TY - JOUR T1 - Regulation and dynamics of force transmission at individual cell-matrix adhesion bonds JF - bioRxiv DO - 10.1101/530469 SP - 530469 AU - Steven J. Tan AU - Alice C. Chang AU - Cayla M. Miller AU - Sarah M. Anderson AU - Louis S. Prahl AU - David J. Odde AU - Alexander R. Dunn Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/01/27/530469.abstract N2 - Integrin-based adhesion complexes link the cytoskeleton to the extracellular matrix (ECM) and are central to the construction of multicellular animal tissues. How biological function emerges from the 10s-1000s of proteins present within a single adhesion complex has remained unclear. We used fluorescent molecular tension sensors to visualize force transmission by individual integrins in living cells. These measurements revealed an underlying functional modularity in which integrin class controlled adhesion size and ECM ligand specificity, while the number and type of connections between integrins and F-actin determined the force per individual integrin. In addition, we found that most integrins existed in a state of near-mechanical equilibrium, a result not predicted by existing models of cytoskeletal force transduction. A revised model that includes reversible crosslinks within the F-actin network accounts for this result, and suggests how cellular mechanical homeostasis can arise at the molecular level. ER -