PT  - JOURNAL ARTICLE
AU  - Calderon, Diego
AU  - Ellis, Andria
AU  - Daza, Riza M.
AU  - Martin, Beth
AU  - Tome, Jacob M.
AU  - Chen, Wei
AU  - Chardon, Florence M.
AU  - Leith, Anh
AU  - Lee, Choli
AU  - Trapnell, Cole
AU  - Shendure, Jay
TI  - <em>Trans</em>MPRA: A framework for assaying the role of many <em>trans</em>-acting factors at many enhancers
AID  - 10.1101/2020.09.30.321323
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.09.30.321323
4099  - http://biorxiv.org/content/early/2020/10/01/2020.09.30.321323.short
4100  - http://biorxiv.org/content/early/2020/10/01/2020.09.30.321323.full
AB  - Gene regulation occurs through trans-acting factors (e.g. transcription factors) acting on cis-regulatory elements (e.g. enhancers). Massively parallel reporter assays (MPRAs) functionally survey large numbers of cis-regulatory elements for regulatory potential, but do not identify the trans-acting factors that mediate any observed effects. Here we describe transMPRA — a reporter assay that efficiently combines multiplex CRISPR-mediated perturbation and MPRAs to identify trans-acting factors that modulate the regulatory activity of specific enhancers.Competing Interest StatementThe authors have declared no competing interest.