PT - JOURNAL ARTICLE AU - Calderon, Diego AU - Ellis, Andria AU - Daza, Riza M. AU - Martin, Beth AU - Tome, Jacob M. AU - Chen, Wei AU - Chardon, Florence M. AU - Leith, Anh AU - Lee, Choli AU - Trapnell, Cole AU - Shendure, Jay TI - <em>Trans</em>MPRA: A framework for assaying the role of many <em>trans</em>-acting factors at many enhancers AID - 10.1101/2020.09.30.321323 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.09.30.321323 4099 - http://biorxiv.org/content/early/2020/10/01/2020.09.30.321323.short 4100 - http://biorxiv.org/content/early/2020/10/01/2020.09.30.321323.full AB - Gene regulation occurs through trans-acting factors (e.g. transcription factors) acting on cis-regulatory elements (e.g. enhancers). Massively parallel reporter assays (MPRAs) functionally survey large numbers of cis-regulatory elements for regulatory potential, but do not identify the trans-acting factors that mediate any observed effects. Here we describe transMPRA — a reporter assay that efficiently combines multiplex CRISPR-mediated perturbation and MPRAs to identify trans-acting factors that modulate the regulatory activity of specific enhancers.Competing Interest StatementThe authors have declared no competing interest.