RT Journal Article SR Electronic T1 TransMPRA: A framework for assaying the role of many trans-acting factors at many enhancers JF bioRxiv FD Cold Spring Harbor Laboratory SP 2020.09.30.321323 DO 10.1101/2020.09.30.321323 A1 Calderon, Diego A1 Ellis, Andria A1 Daza, Riza M. A1 Martin, Beth A1 Tome, Jacob M. A1 Chen, Wei A1 Chardon, Florence M. A1 Leith, Anh A1 Lee, Choli A1 Trapnell, Cole A1 Shendure, Jay YR 2020 UL http://biorxiv.org/content/early/2020/10/01/2020.09.30.321323.abstract AB Gene regulation occurs through trans-acting factors (e.g. transcription factors) acting on cis-regulatory elements (e.g. enhancers). Massively parallel reporter assays (MPRAs) functionally survey large numbers of cis-regulatory elements for regulatory potential, but do not identify the trans-acting factors that mediate any observed effects. Here we describe transMPRA — a reporter assay that efficiently combines multiplex CRISPR-mediated perturbation and MPRAs to identify trans-acting factors that modulate the regulatory activity of specific enhancers.Competing Interest StatementThe authors have declared no competing interest.