RT Journal Article
SR Electronic
T1 <em>Trans</em>MPRA: A framework for assaying the role of many <em>trans</em>-acting factors at many enhancers
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.09.30.321323
DO 10.1101/2020.09.30.321323
A1 Calderon, Diego
A1 Ellis, Andria
A1 Daza, Riza M.
A1 Martin, Beth
A1 Tome, Jacob M.
A1 Chen, Wei
A1 Chardon, Florence M.
A1 Leith, Anh
A1 Lee, Choli
A1 Trapnell, Cole
A1 Shendure, Jay
YR 2020
UL http://biorxiv.org/content/early/2020/10/01/2020.09.30.321323.abstract
AB Gene regulation occurs through trans-acting factors (e.g. transcription factors) acting on cis-regulatory elements (e.g. enhancers). Massively parallel reporter assays (MPRAs) functionally survey large numbers of cis-regulatory elements for regulatory potential, but do not identify the trans-acting factors that mediate any observed effects. Here we describe transMPRA — a reporter assay that efficiently combines multiplex CRISPR-mediated perturbation and MPRAs to identify trans-acting factors that modulate the regulatory activity of specific enhancers.Competing Interest StatementThe authors have declared no competing interest.