RT Journal Article
SR Electronic
T1 <em>Trans</em>MPRA: A framework for assaying the role of many <em>trans</em>-acting factors at many enhancers
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.09.30.321323
DO 10.1101/2020.09.30.321323
A1 Diego Calderon
A1 Andria Ellis
A1 Riza M. Daza
A1 Beth Martin
A1 Jacob M. Tome
A1 Wei Chen
A1 Florence M. Chardon
A1 Anh Leith
A1 Choli Lee
A1 Cole Trapnell
A1 Jay Shendure
YR 2020
UL http://biorxiv.org/content/early/2020/10/01/2020.09.30.321323.abstract
AB Gene regulation occurs through trans-acting factors (e.g. transcription factors) acting on cis-regulatory elements (e.g. enhancers). Massively parallel reporter assays (MPRAs) functionally survey large numbers of cis-regulatory elements for regulatory potential, but do not identify the trans-acting factors that mediate any observed effects. Here we describe transMPRA — a reporter assay that efficiently combines multiplex CRISPR-mediated perturbation and MPRAs to identify trans-acting factors that modulate the regulatory activity of specific enhancers.Competing Interest StatementThe authors have declared no competing interest.