TY - JOUR T1 - Cellular Anatomy of the Mouse Primary Motor Cortex JF - bioRxiv DO - 10.1101/2020.10.02.323154 SP - 2020.10.02.323154 AU - Rodrigo Muñoz-Castañeda AU - Brian Zingg AU - Katherine S. Matho AU - Quanxin Wang AU - Xiaoyin Chen AU - Nicholas N. Foster AU - Arun Narasimhan AU - Anan Li AU - Karla E. Hirokawa AU - Bingxing Huo AU - Samik Bannerjee AU - Laura Korobkova AU - Chris Sin Park AU - Young-Gyun Park AU - Michael S. Bienkowski AU - Uree Chon AU - Diek W. Wheeler AU - Xiangning Li AU - Yun Wang AU - Kathleen Kelly AU - Xu An AU - Sarojini M. Attili AU - Ian Bowman AU - Anastasiia Bludova AU - Ali Cetin AU - Liya Ding AU - Rhonda Drewes AU - Florence D’Orazi AU - Corey Elowsky AU - Stephan Fischer AU - William Galbavy AU - Lei Gao AU - Jesse Gillis AU - Peter A. Groblewski AU - Lin Gou AU - Joel D. Hahn AU - Joshua T. Hatfield AU - Houri Hintiryan AU - Jason Huang AU - Hideki Kondo AU - Xiuli Kuang AU - Philip Lesnar AU - Xu Li AU - Yaoyao Li AU - Mengkuan Lin AU - Lijuan Liu AU - Darrick Lo AU - Judith Mizrachi AU - Stephanie Mok AU - Maitham Naeemi AU - Philip R. Nicovich AU - Ramesh Palaniswamy AU - Jason Palmer AU - Xiaoli Qi AU - Elise Shen AU - Yu-Chi Sun AU - Huizhong Tao AU - Wayne Wakemen AU - Yimin Wang AU - Peng Xie AU - Shenqin Yao AU - Jin Yuan AU - Muye Zhu AU - Lydia Ng AU - Li I. Zhang AU - Byung Kook Lim AU - Michael Hawrylycz AU - Hui Gong AU - James C. Gee AU - Yongsoo Kim AU - Hanchuan Peng AU - Kwanghun Chuang AU - X William Yang AU - Qingming Luo AU - Partha P. Mitra AU - Anthony M. Zador AU - Hongkui Zeng AU - Giorgio A. Ascoli AU - Z Josh Huang AU - Pavel Osten AU - Julie A. Harris AU - Hong-Wei Dong Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/10/02/2020.10.02.323154.abstract N2 - An essential step toward understanding brain function is to establish a cellular-resolution structural framework upon which multi-scale and multi-modal information spanning molecules, cells, circuits and systems can be integrated and interpreted. Here, through a collaborative effort from the Brain Initiative Cell Census Network (BICCN), we derive a comprehensive cell type-based description of one brain structure - the primary motor cortex upper limb area (MOp-ul) of the mouse. Applying state-of-the-art labeling, imaging, computational, and neuroinformatics tools, we delineated the MOp-ul within the Mouse Brain 3D Common Coordinate Framework (CCF). We defined over two dozen MOp-ul projection neuron (PN) types by their anterograde targets; the spatial distribution of their somata defines 11 cortical sublayers, a significant refinement of the classic notion of cortical laminar organization. We further combine multiple complementary tracing methods (classic tract tracing, cell type-based anterograde, retrograde, and transsynaptic viral tracing, high-throughput BARseq, and complete single cell reconstruction) to systematically chart cell type-based MOp input-output streams. As PNs link distant brain regions at synapses as well as host cellular gene expression, our construction of a PN type resolution MOp-ul wiring diagram will facilitate an integrated analysis of motor control circuitry across the molecular, cellular, and systems levels. This work further provides a roadmap towards a cellular resolution description of mammalian brain architecture.Competing Interest StatementThe authors have declared no competing interest. ER -