RT Journal Article
SR Electronic
T1 Cellular Anatomy of the Mouse Primary Motor Cortex
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.10.02.323154
DO 10.1101/2020.10.02.323154
A1 Rodrigo Muñoz-Castañeda
A1 Brian Zingg
A1 Katherine S. Matho
A1 Quanxin Wang
A1 Xiaoyin Chen
A1 Nicholas N. Foster
A1 Arun Narasimhan
A1 Anan Li
A1 Karla E. Hirokawa
A1 Bingxing Huo
A1 Samik Bannerjee
A1 Laura Korobkova
A1 Chris Sin Park
A1 Young-Gyun Park
A1 Michael S. Bienkowski
A1 Uree Chon
A1 Diek W. Wheeler
A1 Xiangning Li
A1 Yun Wang
A1 Kathleen Kelly
A1 Xu An
A1 Sarojini M. Attili
A1 Ian Bowman
A1 Anastasiia Bludova
A1 Ali Cetin
A1 Liya Ding
A1 Rhonda Drewes
A1 Florence D’Orazi
A1 Corey Elowsky
A1 Stephan Fischer
A1 William Galbavy
A1 Lei Gao
A1 Jesse Gillis
A1 Peter A. Groblewski
A1 Lin Gou
A1 Joel D. Hahn
A1 Joshua T. Hatfield
A1 Houri Hintiryan
A1 Jason Huang
A1 Hideki Kondo
A1 Xiuli Kuang
A1 Philip Lesnar
A1 Xu Li
A1 Yaoyao Li
A1 Mengkuan Lin
A1 Lijuan Liu
A1 Darrick Lo
A1 Judith Mizrachi
A1 Stephanie Mok
A1 Maitham Naeemi
A1 Philip R. Nicovich
A1 Ramesh Palaniswamy
A1 Jason Palmer
A1 Xiaoli Qi
A1 Elise Shen
A1 Yu-Chi Sun
A1 Huizhong Tao
A1 Wayne Wakemen
A1 Yimin Wang
A1 Peng Xie
A1 Shenqin Yao
A1 Jin Yuan
A1 Muye Zhu
A1 Lydia Ng
A1 Li I. Zhang
A1 Byung Kook Lim
A1 Michael Hawrylycz
A1 Hui Gong
A1 James C. Gee
A1 Yongsoo Kim
A1 Hanchuan Peng
A1 Kwanghun Chuang
A1 X William Yang
A1 Qingming Luo
A1 Partha P. Mitra
A1 Anthony M. Zador
A1 Hongkui Zeng
A1 Giorgio A. Ascoli
A1 Z Josh Huang
A1 Pavel Osten
A1 Julie A. Harris
A1 Hong-Wei Dong
YR 2020
UL http://biorxiv.org/content/early/2020/10/02/2020.10.02.323154.abstract
AB An essential step toward understanding brain function is to establish a cellular-resolution structural framework upon which multi-scale and multi-modal information spanning molecules, cells, circuits and systems can be integrated and interpreted. Here, through a collaborative effort from the Brain Initiative Cell Census Network (BICCN), we derive a comprehensive cell type-based description of one brain structure - the primary motor cortex upper limb area (MOp-ul) of the mouse. Applying state-of-the-art labeling, imaging, computational, and neuroinformatics tools, we delineated the MOp-ul within the Mouse Brain 3D Common Coordinate Framework (CCF). We defined over two dozen MOp-ul projection neuron (PN) types by their anterograde targets; the spatial distribution of their somata defines 11 cortical sublayers, a significant refinement of the classic notion of cortical laminar organization. We further combine multiple complementary tracing methods (classic tract tracing, cell type-based anterograde, retrograde, and transsynaptic viral tracing, high-throughput BARseq, and complete single cell reconstruction) to systematically chart cell type-based MOp input-output streams. As PNs link distant brain regions at synapses as well as host cellular gene expression, our construction of a PN type resolution MOp-ul wiring diagram will facilitate an integrated analysis of motor control circuitry across the molecular, cellular, and systems levels. This work further provides a roadmap towards a cellular resolution description of mammalian brain architecture.Competing Interest StatementThe authors have declared no competing interest.