RT Journal Article
SR Electronic
T1 Standardized and reproducible measurement of decision-making in mice
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.01.17.909838
DO 10.1101/2020.01.17.909838
A1 The International Brain Laboratory
A1 Valeria Aguillon-Rodriguez
A1 Dora E. Angelaki
A1 Hannah M. Bayer
A1 Niccolò Bonacchi
A1 Matteo Carandini
A1 Fanny Cazettes
A1 Gaelle A. Chapuis
A1 Anne K. Churchland
A1 Yang Dan
A1 Eric E. J. Dewitt
A1 Mayo Faulkner
A1 Hamish Forrest
A1 Laura M. Haetzel
A1 Michael Hausser
A1 Sonja B. Hofer
A1 Fei Hu
A1 Anup Khanal
A1 Christopher S. Krasniak
A1 Inês Laranjeira
A1 Zachary F. Mainen
A1 Guido T. Meijer
A1 Nathaniel J. Miska
A1 Thomas D. Mrsic-Flogel
A1 Masayoshi Murakami
A1 Jean-Paul Noel
A1 Alejandro Pan-Vazquez
A1 Cyrille Rossant
A1 Joshua I. Sanders
A1 Karolina Z. Socha
A1 Rebecca Terry
A1 Anne E. Urai
A1 Hernando M. Vergara
A1 Miles J. Wells
A1 Christian J. Wilson
A1 Ilana B. Witten
A1 Lauren E. Wool
A1 Anthony Zador
YR 2020
UL http://biorxiv.org/content/early/2020/10/04/2020.01.17.909838.abstract
AB Progress in science requires standardized assays whose results can be readily shared, compared, and reproduced across laboratories. Reproducibility, however, has been a concern in neuroscience, particularly for measurements of mouse behavior. Here we show that a standardized task to probe decision-making in mice produces reproducible results across multiple laboratories. We designed a task for head-fixed mice that combines established assays of perceptual and value-based decision making, and we standardized training protocol and experimental hardware, software, and procedures. We trained 140 mice across seven laboratories in three countries, and we collected 5 million mouse choices into a publicly available database. Learning speed was variable across mice and laboratories, but once training was complete there were no significant differences in behavior across laboratories. Mice in different laboratories adopted similar reliance on visual stimuli, on past successes and failures, and on estimates of stimulus prior probability to guide their choices. These results reveal that a complex mouse behavior can be successfully reproduced across multiple laboratories. They establish a standard for reproducible rodent behavior, and provide an unprecedented dataset and open-access tools to study decision-making in mice. More generally, they indicate a path towards achieving reproducibility in neuroscience through collaborative open-science approaches.Competing Interest StatementJ.I.S. is the owner of Sanworks LLC which provides hardware and consulting for the experimental set-up described in this work.