PT - JOURNAL ARTICLE AU - Valerie P. O’Brien AU - Amanda Koehne AU - Julien Dubrulle AU - Armando E. Rodriguez AU - Christina K. Leverich AU - Paul Kong AU - Jean S. Campbell AU - Robert H. Pierce AU - James R. Goldenring AU - Eunyoung Choi AU - Nina R. Salama TI - <em>Helicobacter pylori</em> accelerates KRAS-dependent gastric dysplasia AID - 10.1101/2020.10.06.328518 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.10.06.328518 4099 - http://biorxiv.org/content/early/2020/10/06/2020.10.06.328518.short 4100 - http://biorxiv.org/content/early/2020/10/06/2020.10.06.328518.full AB - More than 80% of gastric cancer is attributable to stomach infection with Helicobacter pylori (Hp), even though the bacterium is not always present at time of diagnosis. Infection is thought to lead to cancer by promoting the accumulation of oncogenic mutations downstream of inflammation; once oncogenic pathways become activated, infection may become dispensable for cancer development. Gastric preneoplastic progression involves sequential changes to the tissue, including loss of parietal cells, spasmolytic polypeptide-expressing metaplasia (SPEM), intestinal metaplasia (IM) and dysplasia. In mice, active KRAS expression recapitulates these tissue changes in the absence of Hp infection. This model provides an experimental system to investigate whether Hp infection has additional roles in preneoplastic progression, beyond initiating inflammation. Mice were assessed by evaluating tissue histology, gene expression changes, the immune cell repertoire, and expression of metaplasia and dysplasia markers. Compared to Hp-/KRAS+ mice, Hp+/KRAS+ mice had i) severe T cell infiltration and altered macrophage polarization; ii) altered expression of metaplasia markers, including increased expression of CD44v9 (SPEM) and decreased expression of TFF3 (IM); iii) more dysplastic (TROP2+) glands; and iv) greater proliferation of metaplastic and dysplastic glands. Hp was able to persistently colonize the stomach during the onset of these tissue changes, and eradication of Hp with antibiotics prevented metaplastic, dysplastic and proliferation marker changes. Collectively, these results suggest that gastric preneoplastic progression differs between Hp+ and Hp-cases, and that sustained Hp infection can promote the later stages of gastric preneoplastic progression, in addition to its established role in initiating chronic inflammation.Competing Interest StatementThe authors have declared no competing interest.