PT  - JOURNAL ARTICLE
AU  - Van Dyke, Krisna
AU  - Mekonnen, Gemechu
AU  - Myers, Chad L.
AU  - Albert, Frank W.
TI  - <em>Trans</em>-acting genetic variation affects the expression of adjacent genes
AID  - 10.1101/2020.10.05.327130
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.10.05.327130
4099  - http://biorxiv.org/content/early/2020/10/07/2020.10.05.327130.short
4100  - http://biorxiv.org/content/early/2020/10/07/2020.10.05.327130.full
AB  - Gene expression differences among individuals are shaped by trans-acting expression quantitative trait loci (eQTLs). Most trans-eQTLs map to hotspot locations that influence many genes. The molecular mechanisms perturbed by hotspots are often assumed to involve “vertical” cascades of effects in pathways that can ultimately affect the expression of thousands of genes. Here, we report that trans-eQTLs can affect the expression of adjacent genes via “horizontal” mechanisms that extend along a chromosome. Genes affected by trans-eQTL hotspots in the yeast Saccharomyces cerevisiae were more likely to be located next to each other than expected by chance. These paired hotspot effects tended to occur at adjacent genes that show coexpression in response to genetic and environmental perturbations. Physical proximity and shared chromatin state, in addition to regulation of adjacent genes by similar transcription factors, were independently associated with paired hotspot effects. The effects of trans-eQTLs can spread among neighboring genes even when these genes do not share a common function. This phenomenon could result in unexpected connections between regulatory genetic variation and phenotypes.Competing Interest StatementThe authors have declared no competing interest.