RT Journal Article
SR Electronic
T1 Sunbeam: an extensible pipeline for analyzing metagenomic sequencing experiments
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 326363
DO 10.1101/326363
A1 Erik L. Clarke
A1 Louis J. Taylor
A1 Chunyu Zhao
A1 Andrew Connell
A1 Jung-Jin Lee
A1 Bryton Fett
A1 Frederic D. Bushman
A1 Kyle Bittinger
YR 2019
UL http://biorxiv.org/content/early/2019/01/29/326363.abstract
AB Background Analysis of mixed microbial communities using metagenomic sequencing experiments requires multiple preprocessing and analytical steps to interpret the microbial and genetic composition of samples. Analytical steps include quality control, adapter trimming, host decontamination, metagenomic classification, read assembly, and alignment to reference genomes.Results We present a modular and user-extensible pipeline called Sunbeam that performs these steps in a consistent and reproducible fashion. It can be installed in a single step, does not require administrative access to the host computer system, and can work with most cluster computing frameworks. We also introduce Komplexity, a software tool to eliminate potentially problematic, low-complexity nucleotide sequences from metagenomic data. Unique components of the Sunbeam pipeline include direct analysis of data from NCBI SRA and an easy-to-use extension framework that enables users to add custom processing or analysis steps directly to the workflow. The pipeline and its extension framework are well documented, in routine use, and regularly updated.Conclusions Sunbeam provides a foundation to build more in-depth analyses and to enable comparisons in metagenomic sequencing experiments by removing problematic low complexity reads and standardizing post-processing and analytical steps. Sunbeam is written in Python using the Snakemake workflow management software and is freely available at github.com/sunbeam-labs/sunbeam under the GPLv3.List of abbreviationsATLASAutomatic Tool for Local Assembly StructuresBAMbinary alignment mapBLASTBasic Local Alignment Search ToolDAGDirected Acyclic GraphEDGEEmpowering the Development of Genomics ExpertiseGPL(GNU) General Public LicenseORF(s)open reading frame(s)SDUSTSymmetric DUSTSRASequence Read ArchiveSURPISequence-based Ultra-Rapid Pathogen IdentificationSVDSpontaneous Vaginal Delivery