TY - JOUR T1 - Neuroanatomical underpinning of diffusion kurtosis measurements in the cerebral cortex of healthy macaque brains JF - bioRxiv DO - 10.1101/2020.07.25.221093 SP - 2020.07.25.221093 AU - Tianjia Zhu AU - Qinmu Peng AU - Austin Ouyang AU - Hao Huang Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/10/07/2020.07.25.221093.abstract N2 - Purpose To investigate the neuroanatomical underpinning of healthy macaque brain cortical microstructure measured by diffusion kurtosis imaging (DKI) which characterizes non-Gaussian water diffusion.Methods High-resolution DKI was acquired from 6 postmortem macaque brains. Neurofilament density (ND) was quantified based on structure tensor from neurofilament histological images of a different macaque brain sample. After alignment of DKI-derived mean kurtosis (MK) maps to the histological images, MK and histology-based ND were measured at corresponding regions of interests characterized by distinguished cortical MK values in the prefrontal/precentral-postcentral and temporal cortices. Pearson correlation was performed to test significant correlation between these cortical MK and ND measurements.Results Heterogeneity of cortical MK across different cortical regions was revealed, with significantly and consistently higher MK measurements in the prefrontal/precentral-postcentral cortex compared to those in the temporal cortex across all 6 scanned macaque brains. Corresponding higher ND measurements in the prefrontal/precentral-postcentral cortex than in the temporal cortex were also found. The heterogeneity of cortical MK is associated with heterogeneity of histology-based ND measurements, with significant correlation between cortical MK and corresponding ND measurements (P <0.005).Conclusion These findings suggested that DKI-derived MK can potentially be an effective noninvasive biomarker quantifying underlying neuroanatomical complexity inside the cerebral cortical mantle for clinical and neuroscientific research.Competing Interest StatementThe authors have declared no competing interest. ER -