RT Journal Article
SR Electronic
T1 Contractile acto-myosin network on nuclear envelope remnants positions human chromosomes for mitosis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 459750
DO 10.1101/459750
A1 Alexander JR Booth
A1 Zuojun Yue
A1 John K Eykelenboom
A1 Tom Stiff
A1 GW Gant Luxton
A1 Helfrid Hochegger
A1 Tomoyuki U Tanaka
YR 2019
UL http://biorxiv.org/content/early/2019/01/30/459750.abstract
AB To ensure proper segregation during mitosis, chromosomes must be efficiently captured by kinetochore microtubules and subsequently aligned on the mitotic spindle. The efficacy of chromosome capture by the mitotic spindle can be influenced by how widely chromosomes are scattered in space. Here, we quantify chromosome-scattering volume (CSV) and find that it is reduced immediately after nuclear envelope breakdown (NEBD) in human cells. The reduction of CSV occurs independently of microtubules and is therefore not an outcome of interactions between chromosomes and the spindle. We find that, prior to NEBD, an acto-myosin network is assembled in a LINC complex-dependent manner on the cytoplasmic surface of the nuclear envelope. This acto-myosin network remains around chromosomes soon after NEBD, and its myosin-II-mediated contraction reduces CSV and facilitates chromosome interaction with spindle microtubules.