%0 Journal Article %A Natalia Orlova %A Dmitri Tsyboulski %A Farzaneh Najafi %A Sam Seid %A Sara Kivikas %A India Kato %A Fiona Griffin %A Arielle Leon %A Quinn L’Heureux %A Kat North %A Jackie Swapp %A Chelsea Nayan %A Nicole Hancock %A Ruweida Ahmed %A Emily Gelfand %A Andrew Cho %A Kyla Mace %A Robert Howard %A Linzy Casal %A Sophie Lambert %A Eric Kenji Lee %A Shiella Caldejon %A Xana Waughman %A Allison Williford %A Marina Garrett %A Doug Ollerenshaw %A Shawn R. Olsen %A Peter Groblewski %A Jérôme Lecoq %A Peter Saggau %T Multiplane Mesoscope reveals distinct cortical interactions following expectation violations %D 2020 %R 10.1101/2020.10.06.328294 %J bioRxiv %P 2020.10.06.328294 %X Cortical columns interact through dynamic routing of neuronal activity. To monitor these interactions, we developed the Multiplane Mesoscope which combines three established microscopy technologies: time-division multiplexing, remote focusing, and random-access mesoscopy. The Multiplane Mesoscope allowed us to study cortical column interactions in excitatory and inhibitory subpopulations in behaving mice. We found that distinct cortical subnetworks represent expected and unexpected events, suggesting that expectation violations modify signal routing across cortical columns, and establishing the Multiplane Mesoscope as a unique platform to study signal routing.Competing Interest StatementThe dual-beam add-on module (D.T., N.O., J.L and P.S.) intellectual property has been licensed to Thorlabs. Inc., by the Allen Institute. %U https://www.biorxiv.org/content/biorxiv/early/2020/10/10/2020.10.06.328294.full.pdf