RT Journal Article
SR Electronic
T1 Using pan RNA-seq analysis to reveal the ubiquitous existence of 5’ end and 3’ end small RNAs
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 444117
DO 10.1101/444117
A1 Xiaofeng Xu
A1 Haishuo Ji
A1 Zhi Cheng
A1 Xiufeng Jin
A1 Xue Yao
A1 Yanqiang Liu
A1 Qiang Zhao
A1 Tao Zhang
A1 Jishou Ruan
A1 Wenjun Bu
A1 Ze Chen
A1 Shan Gao
YR 2019
UL http://biorxiv.org/content/early/2019/01/31/444117.abstract
AB In this study, we used pan RNA-seq analysis to reveal the ubiquitous existence of 5’ end and 3’ end small RNAs. 5’ and 3’ sRNAs alone can be used to annotate mitochondrial with 1-bp resolution and nuclear non-coding genes and identify new steady-state RNAs, which are usually from functional genes. Using 5’, 3’ and intronic sRNAs, we revealed that the enzymatic dsRNA cleavage and RNAi could involve in the RNA degradation and gene expression regulation of U1 snRNA in human. The further study of 5’, 3’ and intronic sRNAs help rediscover double-stranded RNA (dsRNA) cleavage, RNA interference (RNAi) and the regulation of gene expression, which challenges the classical theories. In this study, we provided a simple and cost effective way for the annotation of mitochondrial and nuclear non-coding genes and the identification of new steady-state RNAs, particularly long non-coding RNAs (lncRNAs). We also provided a different point of view for cancer and virus, based on the new discoveries of dsRNA cleavage, RNAi and the regulation of gene expression.