PT  - JOURNAL ARTICLE
AU  - Roberto Navarro Quiroz
AU  - Ana Ligia Scott
AU  - Eric Allison Philot
AU  - Linda Atencio
AU  - Cecilia Fernandez Ponce
AU  - Gustavo Aroca Martinez
AU  - Andres Cadena Bonfanti
AU  - Lorena Gomez Escorcia
AU  - Elkin Navarro Quiroz
TI  - Differential gene expression in the interactome of the Human Dopamine transporter in the context of Parkinson’s disease
AID  - 10.1101/2020.10.12.336388
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.10.12.336388
4099  - http://biorxiv.org/content/early/2020/10/12/2020.10.12.336388.short
4100  - http://biorxiv.org/content/early/2020/10/12/2020.10.12.336388.full
AB  - Background The human dopamine transporter is the main regulator of dopamine tone and an intricate network exists to regulate the expression, conformation, and kinetics of the hDAT. hDAT dysfunction is directly related to Parkinson’s disease. The objective of this work is to evaluate the differential gene expression in the interactome of the Dopamine transporter in the context of Parkinson’s disease.Methods To do this, we evaluated hDAT interaction data in string-db, mint.bio, IntAct, reactome, hprd and BioGRID, subsequently, data was obtained from the differential gene expression of mRNA and miRNAs for this hDAT interactome in the context of PD.Results The analysis of the differential expression changes of genes of the hDAT interactome in tissues of patients with PD compared with tissues of individuals without PD, allowed to identify an expression pattern of 32 components of the hDAT interactome, of which 31 presented a negative change proportion in PD.Conclusions We found a total of 90 miRNAs that could regulate the expression of 27 components of the hDAT interactome, at the same time, 39 components of the hDAT interactome may participate in 40 metabolic pathways. Together, these findings show a systematic effect on the hDAT-mediated dopamine internalization process in patients with Parkinson’s, which would contribute to a greater susceptibility to neuronal oxidative stress in PD patients.Competing Interest StatementThe authors have declared no competing interest.CAMK2ACALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE II-ALPHA; CAMK2ACSKCYTOPLASMIC TYROSINE KINASEEPN1EPS-15-Interacting Protein 1EPS15Epsin-1MTNR1MELATONIN RECEPTOR 1ANEDD4NEURAL PRECURSOR CELL EXPRESSED, DEVELOPMENTALLY DOWNREGULATED 4GEOthe Genetic Expression Omnibus PARK2 E3 ubiquitin-protein ligase parkinPICK1PRKCA-binding proteinhDATThe human dopamine transporterTPPPTUBULIN POLYMERIZATION-PROMOTING PROTEINSLC6A3SOLUTE CARRIER FAMILY 6 (NEUROTRANSMITTER TRANSPORTER, DOPAMINE), MEMBER 3