PT  - JOURNAL ARTICLE
AU  - Janine K Reinert
AU  - Ivo Sonntag
AU  - Hannah Sonntag
AU  - Rolf Sprengel
AU  - Patric Pelzer
AU  - Sascha Lessle
AU  - Michaela Kaiser
AU  - Thomas Kuner
TI  - retroLEAP: rAAV-based retrograde trans-synaptic labeling, expression and perturbation
AID  - 10.1101/537928
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 537928
4099  - http://biorxiv.org/content/early/2019/02/01/537928.short
4100  - http://biorxiv.org/content/early/2019/02/01/537928.full
AB  - Identifying and manipulating synaptically connected neurons across brain regions remains a core challenge in understanding complex nervous systems. RetroLEAP is a novel approach for retrograde trans-synaptic Labelling, Expression And Perturbation. GFP-dependent recombinase (Flp-DOG) detects trans-synaptic transfer of GFP-tetanus toxin heavy chain fusion protein (GFP-TTC) and activates expression of any gene of interest in synaptically connected cells. RetroLEAP overcomes existing limitations, is non-toxic, highly flexible, efficient, sensitive and easy to implement.