RT Journal Article
SR Electronic
T1 retroLEAP: rAAV-based retrograde trans-synaptic labeling, expression and perturbation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 537928
DO 10.1101/537928
A1 Janine K Reinert
A1 Ivo Sonntag
A1 Hannah Sonntag
A1 Rolf Sprengel
A1 Patric Pelzer
A1 Sascha Lessle
A1 Michaela Kaiser
A1 Thomas Kuner
YR 2019
UL http://biorxiv.org/content/early/2019/02/01/537928.abstract
AB Identifying and manipulating synaptically connected neurons across brain regions remains a core challenge in understanding complex nervous systems. RetroLEAP is a novel approach for retrograde trans-synaptic Labelling, Expression And Perturbation. GFP-dependent recombinase (Flp-DOG) detects trans-synaptic transfer of GFP-tetanus toxin heavy chain fusion protein (GFP-TTC) and activates expression of any gene of interest in synaptically connected cells. RetroLEAP overcomes existing limitations, is non-toxic, highly flexible, efficient, sensitive and easy to implement.