RT Journal Article
SR Electronic
T1 Reconstruction of 1,000 projection neurons reveals new cell types and organization of long-range connectivity in the mouse brain
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 537233
DO 10.1101/537233
A1 Johan Winnubst
A1 Erhan Bas
A1 Tiago A. Ferreira
A1 Zhuhao Wu
A1 Michael N. Economo
A1 Patrick Edson
A1 Ben J. Arthur
A1 Christopher Bruns
A1 Konrad Rokicki
A1 David Schauder
A1 Donald J. Olbris
A1 Sean D. Murphy
A1 David G. Ackerman
A1 Cameron Arshadi
A1 Perry Baldwin
A1 Regina Blake
A1 Ahmad Elsayed
A1 Mashtura Hasan
A1 Daniel Ramirez
A1 Bruno Dos Santos
A1 Monet Weldon
A1 Amina Zafar
A1 Joshua T. Dudmann
A1 Charles R. Gerfen
A1 Adam W. Hantman
A1 Wyatt Korff
A1 Scott M. Sternson
A1 Nelson Spruston
A1 Karel Svoboda
A1 Jayaram Chandrashekar
YR 2019
UL http://biorxiv.org/content/early/2019/02/01/537233.abstract
AB Neuronal cell types are the nodes of neural circuits that determine the flow of information within the brain. Neuronal morphology, especially the shape of the axonal arbor, provides an essential descriptor of cell type and reveals how individual neurons route their output across the brain. Despite the importance of morphology, few projection neurons in the mouse brain have been reconstructed in their entirety. Here we present a robust and efficient platform for imaging and reconstructing complete neuronal morphologies, including axonal arbors that span substantial portions of the brain. We used this platform to reconstruct more than 1,000 projection neurons in the motor cortex, thalamus, subiculum, and hypothalamus. Together, the reconstructed neurons comprise more than 75 meters of axonal length and are available in a searchable online database. Axonal shapes revealed previously unknown subtypes of projection neurons and suggest organizational principles of long-range connectivity.