TY - JOUR T1 - Blood-brain barrier dysfunction in aging induces hyper-activation of TGF-beta signaling and chronic yet reversible neural dysfunction JF - bioRxiv DO - 10.1101/537431 SP - 537431 AU - V.V. Senatorov, Jr. AU - A.R. Friedman AU - D.Z. Milikovsky AU - J. Ofer AU - R. Saar-Ashkenazy AU - A. Charbash AU - N. Jahan AU - G. Chin AU - E. Mihaly AU - J.M. Lin AU - H.J. Ramsay AU - A. Moghbel AU - M.K. Preininger AU - C.R. Eddings AU - H.V. Harrison AU - R. Patel AU - Y. Shen AU - H. Ghanim AU - H. Sheng AU - R. Veksler AU - P.H. Sudmant AU - A. Becker AU - B. Hart AU - M.A. Rogawski AU - A. Dillin AU - A. Friedman AU - D. Kaufer Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/02/01/537431.abstract N2 - Aging involves a decline in neural function that contributes to cognitive impairment and disease. However, the mechanisms underlying the transition from a young-and-healthy to aged-and-dysfunctional brain are not well understood. Here, we report breakdown of the vascular blood-brain barrier (BBB) in aging humans and rodents, which begins as early as middle age and progresses to the end of the lifespan. Gain-of-function and loss-of-function manipulations show that this BBB dysfunction triggers hyperactivation of transforming growth factor β (TGFβ) signaling in astrocytes, which is necessary and sufficient to cause neural dysfunction and age-related pathology. Specifically, infusion of the serum protein albumin into the young brain (mimicking BBB leakiness) induced astrocytic TGFβ signaling and an aged brain phenotype including aberrant electrocorticographic activity, vulnerability to seizures, and cognitive impairment. Furthermore, conditional genetic knockdown of astrocytic TGFβ receptors, or pharmacological inhibition of TGFβ signaling, reversed these symptomatic outcomes in aged mice. Finally, we found that this same signaling pathway is activated in aging human subjects with BBB dysfunction. Our study identifies dysfunction in the neurovascular unit as one of the earliest triggers of neurological aging, and demonstrates that the aging brain may retain considerable latent capacity which can be revitalized by therapeutic inhibition of TGFβ signaling. ER -