RT Journal Article
SR Electronic
T1 Reconfiguring primase DNA-recognition sequences by using a data-driven approach
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.09.29.317842
DO 10.1101/2020.09.29.317842
A1 Soffer, Adam
A1 Ifrach, Morya
A1 Ilic, Stefan
A1 Afek, Ariel
A1 Vilenchik, Dan
A1 Akabayov, Barak
YR 2020
UL http://biorxiv.org/content/early/2020/10/14/2020.09.29.317842.abstract
AB DNA–protein interactions are essential in all aspects of every living cell. Understanding of how features embedded in the DNA sequence affect specific interactions with proteins is challenging but important, since it may contribute to finding the means to regulate metabolic pathways involving DNA–protein interactions. Using a massive experimental benchmark dataset of binding scores for DNA sequences and a machine learning workflow, we describe the binding to DNA of T7 primase, as a model system for specific DNA–protein interactions. Effective binding of T7 primase to its specific DNA recognition se-quences triggers the formation of RNA primers that serve as Okazaki fragment start sites during DNA replication.Competing Interest StatementThe authors have declared no competing interest.