RT Journal Article
SR Electronic
T1 The ubiquitylome of developing cortical neurons
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.10.13.337782
DO 10.1101/2020.10.13.337782
A1 Shalini Menon
A1 Dennis Goldfarb
A1 Emily M. Cousins
A1 M. Ben Major
A1 Stephanie L. Gupton
YR 2020
UL http://biorxiv.org/content/early/2020/10/14/2020.10.13.337782.abstract
AB TRIM9 and TRIM67 are neuronally-enriched E3 ubiquitin. Both genes are required for neuronal morphological responses to the axon guidance cue netrin-1. For example, our previously published work demonstrated that the actin polymerase VASP and the netrin receptor DCC exhibit TRIM9 dependent ubiquitylation that is lost upon netrin stimulation. Deletion of either gene in the mouse results in subtle neuroanatomical anomalies yet overt deficits in spatial learning and memory. Despite their role in neuronal form and function, the identity of few TRIM9 or TRIM67 substrates are known. Here we performed ubiquitin remnant profiling approach in cultured wildtype and knockout murine embryonic cortical neurons to identify ubiquitylated peptides and proteins, with the ultimate goal of identifying substrates of TRIM9 and TRIM67 that exhibited reduced ubiquitylation in the absence of the ligase. This work reveals the ubiquitylome of developing cortical neurons.Competing Interest StatementThe authors have declared no competing interest.