PT  - JOURNAL ARTICLE
AU  - Nicholas L. Turner
AU  - Thomas Macrina
AU  - J. Alexander Bae
AU  - Runzhe Yang
AU  - Alyssa M. Wilson
AU  - Casey Schneider-Mizell
AU  - Kisuk Lee
AU  - Ran Lu
AU  - Jingpeng Wu
AU  - Agnes L. Bodor
AU  - Adam A. Bleckert
AU  - Derrick Brittain
AU  - Emmanouil Froudarakis
AU  - Sven Dorkenwald
AU  - Forrest Collman
AU  - Nico Kemnitz
AU  - Dodam Ih
AU  - William M. Silversmith
AU  - Jonathan Zung
AU  - Aleksandar Zlateski
AU  - Ignacio Tartavull
AU  - Szi-chieh Yu
AU  - Sergiy Popovych
AU  - Shang Mu
AU  - William Wong
AU  - Chris S. Jordan
AU  - Manuel Castro
AU  - JoAnn Buchanan
AU  - Daniel J. Bumbarger
AU  - Marc Takeno
AU  - Russel Torres
AU  - Gayathri Mahalingam
AU  - Leila Elabbady
AU  - Yang Li
AU  - Erick Cobos
AU  - Pengcheng Zhou
AU  - Shelby Suckow
AU  - Lynne Becker
AU  - Liam Paninski
AU  - Franck Polleux
AU  - Jacob Reimer
AU  - Andreas S. Tolias
AU  - R. Clay Reid
AU  - Nuno Maçarico da Costa
AU  - H. Sebastian Seung
TI  - Multiscale and multimodal reconstruction of cortical structure and function
AID  - 10.1101/2020.10.14.338681
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.10.14.338681
4099  - http://biorxiv.org/content/early/2020/10/14/2020.10.14.338681.1.short
4100  - http://biorxiv.org/content/early/2020/10/14/2020.10.14.338681.1.full
AB  - We present a semi-automated reconstruction of L2/3 mouse primary visual cortex from 3 million cubic microns of electron microscopic images, including pyramidal and inhibitory neurons, astrocytes, microglia, oligodendrocytes and precursors, pericytes, vasculature, mitochondria, and synapses. Visual responses of a subset of pyramidal cells are included. The data are being made publicly available, along with tools for programmatic and 3D interactive access. The density of synaptic inputs onto inhibitory neurons varies across cell classes and compartments. We uncover a compartment-specific correlation between mitochondrial coverage and synapse density. Frequencies of connectivity motifs in the graph of pyramidal cells are predicted quite accurately from node degrees using the configuration model of random graphs. Cells receiving more connections from nearby cells exhibit stronger and more reliable visual responses. These example findings illustrate the resource’s utility for relating structure and function of cortical circuits as well as for neuronal cell biology.Competing Interest StatementTM and HSS disclose financial interests in Zetta AI LLC. JR and AST disclose financial interests in Vathes LLC.